FluoQ / Multiplex Unlocking

Start with a clean background

FluoQ fluorescence quenching system efficiently eliminates prior-round fluorescence signal and autofluorescence, unlocking the full picture of complex biological structures

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Efficient Quenching

Rapidly and thoroughly removes fluorescence signal to prevent channel crosstalk, ensuring a pristine background for every imaging round and improving data reliability

Hyperplex Imaging

Breaks free from spectral channel limits with multi-round staining cycles, enabling hyperplex fluorescence labeling and multi-dimensional observation of complex biology

Sample Compatibility

Compatible with paraffin sections, frozen sections, cell crawls, thick tissue sections, and more — covering pathology, immunology, and broader research scenarios

Every imaging round, starting on a clean background

Residual signal is the hardest variable to control in multi-round imaging. FluoQ delivers complete quenching after each round — autofluorescence included — so the next antibody incubation begins on a genuinely clean background

01
Image current round
Capture high-quality fluorescence images of the current round's targets using PanoCube, PanoFlow, or other Meca imaging instruments — ensuring data completeness
02
FluoQ quenches efficiently
Load the sample into FluoQ, add the matched quenching kit. The system runs the automated quenching protocol, thoroughly clearing the round's fluorescence signal
03
Prepare next round
With a pristine background, begin the next round of fluorescent antibody labeling on the same sample — no residual interference, sharper signal interpretation
04
Loop to hyperplex
Repeat the stain–image–quench cycle to accumulate dozens of markers on a single sample, unlocking the full picture of complex biological structures

Round 1 stained section: CREPT, CD3, CD20

Round 2 stained section: CD56, CD68, SMA, CK19, CD31

Section overlay: registered by the DAPI channel to generate a hyperplex image

30+ markers, one section

The cap on fluorescent labels has never been the antibody — it's bleaching. FluoQ makes the same section reusable round after round. Each cycle lets you reincubate with a fresh antibody panel; markers stack linearly with cycles. Experiments that once needed multiple sections now fit on one

Find your experiment

Multi-round Immunofluorescence (mIF)

Continuously label a dozen-plus protein markers on a single tissue section, achieving information density beyond conventional multicolor while preserving full spatial context

Spatial Transcriptomics Companion Imaging

Pair with platforms like Visium and Xenium to add a protein-level spatial layer to transcriptomic data; images plug directly into co-registration workflows

High-yield Use of Rare Samples

Biopsies, surgical margins, and other irreplaceable samples often yield only one section. FluoQ's low-damage chemistry lets a single section carry far more dimensions of measurement

Neural Circuit Analysis

High autofluorescence has long been the bottleneck for multicolor imaging in neural tissue. FluoQ's built-in autofluorescence suppression markedly improves SNR in brain sections

Drug Target Spatial Validation

Simultaneously assess target expression, immune infiltration, and tissue architecture on a single section — a complete spatial evidence chain for candidate-drug mechanisms

Large-cohort Tissue Microarrays (TMA)

Automated workflow makes multi-round imaging of hundred-case TMAs operationally feasible, with standardized acquisition that reduces batch variability for downstream analysis

Make cyclic staining your default workflow

Start from your experimental needs — let's discuss what multiplex imaging can unlock for you